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Running Head: Shorter Version of Full Title (Double click here to change)




Full Title Should Say What This Lab Report


Is Presenting as a Conclusion (Determination of Unknown Bacter # as Bacteria Name)


John Smith


Los Angeles City College


Microbiology 20


Dr. David J. Sanchez


June 2, 2012

Abstract

Please delete all these extra instructions in blue and replace with your text. The abstract should be written here and only one page for this part, start a new page for the next part. The first sentence is what the general topic is. The second sentence is what the research question is. The third sentence is a summary of the method we used. The fourth sentence is what results were found. The fifth sentence is what are the implications of such a finding. One point per sentence for a total of 5 points for the abstract. Notice this is on a separate sheet of paper.


Centered Full Title from the Cover Page

Begin with a short introduction. Just a few sentences is fine. Focus on the goal of this set of experiments: to determine the identity of an unknown bacteria.

Scientific writing is not exciting. In this paragraph, you will summarize your findings. Explain the major characteristics of the bacteria: like “Unknown bacteria # is Gram +/- (Endospore or Acid Fast positive if it is gram positive) and that it ferments whateever it ferments”. Say that “We have determined that Unknown bacteria #, is _____” as the last sentence.

Methods

This paragraph is a detailed explanation of the experiemental setup of determining an unknown bacteria. DO NOT PUT IT DETAILS LIKE: “Flamed a loop” or “Added three drops of oxidase”. In stead say the outline of methods for how to determine the unknown uncluding 1) Streaked bacteria for a single colony (we did not do it, but that is the first step) 2) isolate a pure culture, 3) gram stain, 3a) if you had a gram + then what other stains did you do, 4) Phenol Red Tests, 5) Other metabolic tests. Everything must be named, and generally explained what you did. For the gram stain, it is sufficient to say you did the Gram stain by normal procedures.

This will take about 3 paragraphs at least. Please be sure to include if the items were tubes or plates.

Results

This will be the longest part of the report including your individual observerations from what color the bacteria is on the tube to each tube color before and after use. So the best part is to start the section with something like what the test is in bold (Gram Stain.) and a period. Show each observation and what that result means. Is it Gram+/-, does it ferment the sugar, etc. This will take several paragraphs.

Finally, include a table with your resutls. It has three collums, first with the test or stain name, next with the result (positive, negative, red, yellow, etc), thrird collumn with a summarized result outcome (Gram +, Ethanol ferments lactose, etc)

Every test must be here, with include, Staining, Phenol Reds, Simmons Citrate, Kliger’s Iron Agar, Spirit Blue, Starch, Skim Milk, Catalse, Oxidase Tests. If you did not do one, email me and ask for your bacteria’s results, if you need help determining your bacteria than email me with you number and what you are confused about.

Conclusion

The conclusion will begin with a reitteration of the results as you determine what your unknown bacteria is. For example, the gram staining, leads the reader to the possibilty of addiitonal staining or that what is next.

State definitely your conclusion.

Finally, use at least two paragraphs to explain how this bacteria causes disease if it does. If it does not explain how it can be an opprotunisitc pathogen and cause disease in immunocompromised hosts. If it does not cause a disease, do other similar bacteria cause a disease that you can hypothesize would be similar in an immunocompromised host. Also, detail what organs or locations of the body could be affected. Is this bacteria part of the normal flora/microbiota?

References

Fill in references such as (Notice this is a new page!) You must have a minimum of 4 references (3 referneces that you must have in your reference section are here, in addition, you will need to find at least one reference about your genus/species of bacteria beyond Wikipedia. Wikipedia may be here, but it will be a 5th reference): The Holt reference is for the charts to determine the bacteria, The Leboffe is for how the tests look (if you lab book you should use if you forgot what the tests do) and the textbook can be used to find more information on the bacteria)

Holt, J.G., ed (1994) Bergey’s Manual of Determinative Bacteriology, 9th ed. Lippincott,

Williams and Wilkins .

Leboffe, M.J., Pierce, B.E. (2005) A Photographic Atlas for the Microbiology Laboratory.

Morton Publishing Company.

Tortora, G.J., Funke, B. R., Case, C.L. (2009) Microbiology: An Introduction. Benjamin-

Cummings Publishing Company.



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