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Proposal for New Course
BIO 205L Microbiology Lab
College of Engineering, Forestry and Natural Sciences
BIO 205L Microbiology Lab
3 Clock hours, 1 credit hour
Laboratory Manager, Sr.
BIO 181 or BIO 181H
Examines the function of organisms, aspects of microbial diversity, pathogenic microorganisms, disease mechanisms and immune response.
This is the lab portion of BIO 205 Microbiology. This course will emphasize hands on experience with many of the diverse processes that pertain to the microbial community which are addresses in the BIO 205 lecture. The labs are not necessarily aligned with the lecture material but do emphasize similar topics. The content requires students to be involved in the experimental design process. This includes technical expertise, data collection and organization, problem solving in a team environment and presentation of results.
Student Learning Expectations/Outcomes
Students will demonstrate proper wet lab technique which include but are not limited to: aseptic technique, dilution series, wet mounts, microscope use and care, etc.
Students will learn how to collect and organize data.
Students will learn to work in a team environment.
Students will learn to problem solve results and present written results.
Students will demonstrate professionalism which includes, but is not limited to: paying attention, following directions, following safe laboratory procedures, wearing your lab coat, coming to lab prepared and on time, etc.
Required published reading materials for the BIO 205 laboratories include:
• Fundamental Experiments and Techniques in Microbiology; an "in-house" publication.
• A Photographic Atlas for the Microbiology Laboratory; M.J. Leboffe and B.E. Pierce, 3rd Edition, 2005.
• Writing Papers in the Biological Sciences; V.E. McMillan, 4th Edition, 2001.
Week 1 BIO 205 Orientation and Exercise 1: Microscopy
Week 2 Exercise 2 Staining, Begin Purification, Morphology
Week 3 Exercise 3 Staining: Sterile Technique
Week 4 Staining Week
Week 5 Exercise 4 Staining Continued: Sterile Technique & Gram Unknown
Week 6 Exercise 5 Enumeration
Week 7 Exercise 6 Data Analysis
Week 8 Exercise 7 Biochemical Tests
Week 9 Exercise 8 Bacteriophage
Week 10 Exercise 9 Transformation and Bacteriophage Worksheet
Week 11 Exercise 10 UV Analysis of Microorganisms and TransformationWorksheet
Week 12 Exercise 11 Practical Review
Week 13 Lab Practical
Week 14 Lab Cleanup and Evaluations
Week 15 Finals
Assesment of Student Learning Outcomes
Quizzes, written and technical
(300 points possible)
8 Work Sheets (100 points total)
Phage One-step Growth 10 points
Transformation 10 points
Others 80 points
Lab Practical 50 points
Technique 40 points
Professionalism 40 points
Gram Stain Unknown 40 points
7 quizzes (5 pts/quiz; you can drop one) 30 points
Total 300 points
A= 90 and above, B=89-80, C=79-70, D=69-60,F = 59 and below
You will be expected to come back to the laboratory at times other than your regularly scheduled lab period to record the results of various experiments or to remove cultures that are incubating at 37°C and place them in the cold room.
Attendance is mandatory and roll call will be taken during each lab. After the first lab period there will be no unexcused absences allowed. The penalty for any unexcused absence may result in the student being administratively dropped from the course.
There will be seven (7) unannounced quizzes administered during the semester covering material for the upcoming lab or other material that your laboratory instructor may include. You will be allowed to drop one quiz grade. Missed quizzes cannot be made up.
If you miss a laboratory period that included an experiment for which you must prepare a worksheet, you will lose ten (10) points. Since enrollment in the laboratories is at maximum, except under extraordinary circumstances where you have made previous arrangements with your laboratory instructor, it is not feasible to attend a laboratory section other than the one in which you are enrolled.
Academic Dishonesty (including, but not limited to, plagiarism and cheating): See "Academic Integrity" in the on-line Student Handbook at http://www4.nau.edu/stulife/StudentHandbook/AcademicPolices.htm. These penalties for plagiarism are up to and including an “F” in the lab and/or lecture. Webster's Tenth New Collegiate Dictionary defines the word plagiarize as ": to steal and pass off (the ideas or words of another) as one's own: use (a created production) without crediting the source ~ vi : to commit literary theft : present as new and original an idea or product derived from an existing source." Microbiology laboratory experiments are often done in groups of one, two or more students, discussion and examination of methodology and results between students with respect to the laboratory experiment is encouraged, but this collaboration stops when any written component is required. Each student will write independently his or her own report.
From: Linda Sue Fitchett-Hewitt
Sent: Thursday, January 27, 2011 11:42 AM
To: Stuart S Galland
Cc: Peggy Ellen Pollak
Subject: RE: BIO 205 syllabus
This is the correct grading scale. A=90, B=89-80, C=79-70, D=69-60,F =59 below-50.
From: Peggy Ellen Pollak
Sent: Thursday, January 27, 2011 11:36 AM
To: Linda Sue Fitchett-Hewitt
Subject: FW: BIO 205 syllabus
Will you confirm or modify the grading in 205L and send the info to Scott please?
Peggy E. Pollak
Dept. Biological Sciences
Northern Arizona University
Flagstaff, AZ 86011-5640
From: Stuart S Galland
Sent: Thursday, January 27, 2011 10:03 AM
To: Peggy Ellen Pollak
Cc: William Sylvester Allred Jr
Subject: FW: BIO 205 syllabus
HI Peggy and Syl,
I guess Rosemary is out of town?
The BIO 205L syllabus needs to have the grading scale added, and I am happy to do it if you can confirm (A=90, B=89-80, etc) and approve.
Curriculum Process Associate
Office of the Associate Vice Provost
for Academic Affairs
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