ANTIMUTAGENESIS AND CHEMOPREVENTION
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Responsible teacher: Vuković-Gačić S. Branka
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Course status: module elective
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ECTS: 5
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Mutagens: physical, chemical and biological. Molecular mechanisms of DNA repair in procayotes and eucaryotes. Repair of DNA molecule and mutagenesis. Мutagenesis and carcinogenesis. Aging and mutation related degenerative deseases. Antimutagenesis and anticarcinogenesis. Mechanisms of antimutagenesis, desmutagens and bioantimutagens. Procaryotic and eucaryotic models for detection of antimutagenic potential and mechanisms of antimutagenesis. Primary chemoprevention, extracellular and intreacellular. Secundary chemoprevention. Inhibition of tumor progression and angiogenesis. Inhibition of invasion and metastase.
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Literature:
Friedberg, C.E., Walker, C.G., Siede, W., Wood, R. D., Schults, R.A, Ellenberger, T., (2006), DNA repair and mutagenesis, ASM Press, Washington, USA
Watson, J. D., Baker, T. A. Bell, S. P. Gann, A., Levine M., Losick, R. (2007), Molecular biology of the gene, 6th Ed, The Benjamin/Cummings Publishing Company, Inc.
Mechanistic Approaches to Chemoprevention of Mutation and Cancer (2005) Eds. L. Ferguson et al., Mutation Res., 591, 1-310.
Vuković-Gačić, B., Simić, D., Knežević-Vukčević, J., (2006), Escherichia coli assay system for detection of plant antimutagens and their mechanisms of action, In: Topical Issues in Applied Microbiology and Biotechnology, Ed. L. Verschaeve, Research SignPost, India.
Scientific publications.
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Lectures and tutorials: 3
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Projects, seminars: 2
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Grading (maximum 100)
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Seminars 30
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Project presentations 30
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Oral examination 40
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DIVERSITY, ECOLOGY AND EVOLUTION OF MICROORGANISMS
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Responsible teacher: Knežević-Vukčević B. Jelena
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Course status: module elective
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ECTS: 5
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The concept of species and nomenclature of microorganisms. Biochemical, physiological and ecological diversity of microorganisms. Diversity and stability of microbial communities. Interactions in microbial communities. Microbial interactions with plants and animals. Interactions of microorganisms with pollutants in the biosphere. Molecular ecology and genomics of microorganisms. Theories on the origin of microorganisms. Methods for determining the phylogenetic relationships. Molecular taxonomy. Phylogenetic tree and the evolution of prokaryotes. Classification and representative groups of prokaryotes. Evolution and classification of viruses.
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Literature:
Madigan, M.T., Martinko, J.M., (2006) Brock Biology of Microorganisms, 11th Ed., Prentice Hall Int.
Atlas, M. R., Bartha, R., (1998), Microbial Ecology-Fundamentals and Applications, 4th Ed., Addison Wesley Longman, Inc.
Molecular Microbial Ecology (2005), Eds., A. M. Osborn and C.J. Smith, Taylor & Francis Group.
Microbial Phylogeny and Evolution, (2005) Ed. Saap J., Oxford University Press.
Bergey’s Manual of Systematic Bacteriology, (2001-), 2nd ed. Ed. G.M. Garrity, Springer.
Review articles.
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Lectures and tutorials: 3
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Projects, seminars: 2
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Grading (maximum 100)
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Seminars 30
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Project presentations 30
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Oral examination 40
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METHODS IN MICROBIOLOGY (ADVANCED COURSE)
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Responsible teachers: Knežević-Vukčević B. Jelena, Vuković-Gačić S. Branka, Stanković M. Slaviša
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Course status: compulsory module
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ECTS: 8
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Laboratory safety. Methods for studying morphology and ultrastructure of microorganisms. Biophisical and biochemical growth factors. Isolation of pure culture. Enrichment techniques. Selection techniques. Methods for culturing and preservation of microorganisms. Growth measurmenst. Methods for studying bacteriophages: isolation, multiplication, purification and enumeration. Interaction with host bacteria. Experimental methods in bacterial genetics: mutagenesis, isolation and characterization of mutants. Transformation. Conjugation. Transduction. Construction of strains. Isolation, analysis and construction of plasmids. Identification of microorganisms. Methods for molecular characterization of microorganisms. Clasical and molecular methods in microbial ecology. Determination of microorganisms and their activity in natural samples. Microbial models and techniques for determination of biological activities of substances. Microbial tests for detection of mutagens and antimutagens.
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Literature:
Rayburn, S.R. (1990) The Foundations of Laboratory Safety, Springer-Verlag.
Madigan, M.T., Martinko, J.M., (2006) Brock Biology of Microorganisms, 11th Ed., Prentice Hall Int.
Manual of Methods for General Bacteriology (1981) Eds. Gerhardt et al., AMS, Washington DC, USA.
Snyder, L. and Champness, W. (2007) Molecular Genetics of Bacteria. 3rd Ed., ASM Press, USA.
Birge, E. A., (2000), Bacterial and Bacteriophage Genetics, 4th Ed., Springer-Verlag N.Y. Inc.
Escherichia coli and Salmonella typhimurium cellular and molecular biology, Vols. 1 and 2, Ed. Neidhardt, F.C. (1987) ASM Press, USA.
Friedberg, C.E., Walker, C.G., Siede, W., Wood, R. D., Schults, R.A, Ellenberger, T. (2006) DNA repair and mutagenesis, ASM Press, USA.
Maloy, S.R. (1990) Experimental Techniques in Bacterial Genetics, Jones and Bartlett Publ., Boston, USA.
Molecular Microbial Ecology (2005), Ed., A. M. Osborn and C.J. Smith, Taylor & Francis Group.
Manual of Environmental Microbiology (2002), 2nd ed. Eds C.J. Hurst et all., ASM Press, USA.
Bergey’s Manual of Systematic Bacteriology, (2001-), 2nd ed. Ed. G.M. Garrity, Springer.
Review articles.
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Lectures and tutorials: 4
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Experiments, projects, seminars: 4
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Grading (maximum 100)
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Seminars 20
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Project presentations 20
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Presentations of rexperimental results 20
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Oral examination 40
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MICROBIOLOGY OF AQUATIC ECOSYSTEMS (PhD-HYD-E4)
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Elective course
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Responsible teacher: Stanković M. Slaviša
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6 ECTS
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The structure and activities of microbial communities in groundwater and surface waters. Stratification. Microorganisms of great depth - extremophiles. Microbiological transformations in waters. Biodegradation of oil, xenobiotics, pesticides, synthetic polymers, transformations of metals. Microorganisms in the production of oil. Microorganisms as a source of biodegradable products and clean energy. Bacteria as indicators of water quality. Eutrophication and saprobity of water - bioindicators. Autopurification. Microbial interactions with pollutants. Purification of polluted waters. The role of microorganisms in the sedimentation of iron and manganese in underground waters. Ecological and sanitary aspects of water analysis. Genotoxic monitoring of water. Integrated approach to monitoring of water.
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Literature:
Atlas, R. M. & Bartha, R. (1998). Microbial Ecology – Fundamentals and Applications, Fourth Ed.. Addison Wesley Longman, Inc.;
Saap, J. (Ed.) (2005). Microbial Phylogeny and Evolution. – Oxford University Press.;
Osborn, A. M. & Smith, C.J. (Eds) (2005). Molecular Microbial Ecology. – Taylor & Francis Group.;
Hurst, C. J. et al. (2002). Manual of Environmental Microbiology, second edition. – ASM Press.
Review articles.
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Lectures and tutorials: 3
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Projects, seminars: 2
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Grading (maximum 100)
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Seminars 30
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Project presentations 30
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Oral examination 40
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MICROBIOLOGY OF DIFFERENT HABITATS
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Responsible teacher: Stanković M. Slaviša
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Course status: module elective
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ECTS: 5
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The structure and activities of microbial communities in different soil layers. Rhizosphere. Interactions of microorganisms and plants. Biotransformation of plant residues. Formation and decomposition of humus. Microorganisms of great depth. The effect of acid rain on microbial processes in soil. Bioremediation of contaminated soil, biodegradation of oil, xenobiotics, pesticides, synthetic polymers, transformations of metals. Production of healthy food. Biofertilization. Biopesticides. Other aspects of microbial protection of crops. Exploitation of natural resources using microorganisms. Microorganisms in the production of oil. Microorganisms as a source of biodegradable products and clean energies.
The structure and activities of of microbial communities in groundwater and surface waters. Stratification. Microorganisms of great depth - extremophiles. Microbiological transformations in waters. Bacteria as indicators of water quality. Eutrophication and saprobity of water - bioindicators. Autopurification. Microbial interactions with pollutants. Purification of polluted waters. The role of microorganisms in the sedimentation of iron and manganese in underground waters. Ecological and sanitary aspects of water analysis. Genotoxic monitoring of water. Integrated approach to monitoring of water.
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Literature:
Microbial Ecology-Fundamentals and Applications, 4th Ed., Addison Wesley Longman, Inc. Microbial Phylogeny and Evolution, (2005) Ed. Saap J., Oxford University Press.
Molecular Microbial Ecology (2005), Ed., A. M. Osborn and C.J. Smith, Taylor & Francis Group. Manual of Environmental Microbiology (2002), Eds C.J. Hurst et all., 2nd Ed., ASM Press.
Review articles.
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Lectures and tutorials: 3
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Projects, seminars: 2
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Grading (maximum 100)
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Seminars 30
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Project presentations 30
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Oral examination 40
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MICROORGANISMS IN BIOTECHNOLOGY AND ENVIRONMENTAL PROTECTION
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Responsible teacher: Knežević-Vukčević B. Jelena
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Course status: module elective
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ECTS: 5
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Topics: Principles of biotechnology. Application of microorganisms in different industrial processes. Selection of microorganisms from natural samples. Microbial models and tests for biological activities. Genetic manipulations for construction of industrial strains. Optimization of culture conditions. Fermentations. Scale-up. Microorganisms in exploitation of natural resources. Microorganisms as a source of biodegradable products and clean energies. Microorganisms in bioremediation, biodegradation, biofertilization and biocontrol. Microorganisms in genetic engineering and construction of transgenic animals and plants.
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Literature:
Barnum, S.R. (2005), Biotechnology, An Introduction, 2nd Ed., Thomson Brooks/Cole, USA.
Madigan, M.T., Martinko, J.M., (2006) Brock Biology of Microorganisms, 11th Ed., Prentice Hall Int.
Miesffeld, R.L., (1999), Applied molecular genetics, Wiley-Liss, Inc.
Taylor, J. (1990), Microorganisms and Biotehnology, MacMillan Education LTD.
Walker, G. M. (1999) Yeast Physiology and Biotechnology, John Wily and Sons Ltd.
Review articles.
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Lectures and tutorials: 3
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Projects, seminars: 2
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Grading (maximum 100)
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Seminars 30
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Project presentations 30
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Oral examination 40
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