B. Sc. II paper No Title Marks V applied Microbiology 100 VI immunology & clinical microbiology 100 VII practical 100 VIII practical 100 B. Sc. III




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НазваниеB. Sc. II paper No Title Marks V applied Microbiology 100 VI immunology & clinical microbiology 100 VII practical 100 VIII practical 100 B. Sc. III
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DR. BABASAHEB AMBEDKAR MARATHWADA UNIVERSITY AURANGABAD


Syllabus


of



B.Sc. [ Microbiology ]


DR. BABASAHEB AMBEDKAR MARATHWADA UNIVERSITY AURANGABAD

B.Sc. Microbiology

Effective progressively from June, 2008.


B.Sc. I


Paper No Title Marks

I Fundamentals of Microbiology 100

II Basic Biochemistry & Microbial Physiology 100

III Practical 100

IV Practical 100

B.Sc. II



Paper No Title Marks


V Applied Microbiology 100

VI Immunology & clinical microbiology 100

VII Practical 100

VIII Practical 100

B.Sc. III



Paper No Title Marks


IX Microbial genetics & Recombinant DNA Technology 100

X Microbial Metabolism & Industrial Microbiology 100

XI Practical 100

XII Practical 100


Total marks 1200




B.Sc. First Year




Paper I .Fundamentals of Microbiology




Unit – I 1 Scope & relevance of Microbiology (15)


i) Definition & concepts

ii) Types of microorganism

iii) Distribution of Microorganisms in nature

2. Development of Microbiology as a Scientific Discipline


1.1 History

i) Early observation of microorganisms

ii) Spontaneous generation conflict : Contribution of scientists

iii) Recognition of the microbial role in diseases. Koch’s postulates

iv) Recognition of microbial role in fermentations.

v) Discovery of microbial effects on organic and inorganic matter.

vi) Pure culture concept.

vii) Aseptic surgery

Unit – 2 General characteristics of microorganisms. (15)

2.1 General principles ( Brief )


i) Taxonomic rank

ii) Classification system

iii) Numerical taxonomy

iv) Major characteristics used in taxonomy. Morphological, Physiological, Metabolic, Etiological, Genetic analysis, Molecular Characteristics .

Compositions of proteins, composition of nucleic acids, hybridization, nucleic acid sequencing, identification of organisms based on 16srRNA sequencing.

v) Bergey’s manual of determinative Bacteriology, General characteristics enlisting 19 parts with major characters & examples.

2.2 General characteristics and classification of Viruses

2.3 General characters of Fungi ( including yeasts )

2.4 General characters of Actinomycetes

2.5 General characters of algae

2.6 General characteristics of Mycoplasma and Rickettssia.

2.7 Introduction to Gnotobiology/ axenic life/ space microbiology.

Unit – 3 : 3.1 Microscopy (15)


3.1 Properties of light

i) Definitions : Magnification, resolving power, depth of focus, focal length, numerical aperture.

ii) Objectives Low , high & oil immersion.

iii) Oculars : function, Huygenian, Ramsden, Hyperplane & compensating.

iv) Condensers ; Abbe, variable focus cordiod, parabolic & their functions.

v) Iris diaphragm.

3.2 Principles, construction using ray diagram, application and comparative study of :

i) Comound Microscope

ii) Electron Microscope

3.3 Principles, ray diagram & applications.

i) Phase contrast microscope.

ii) Dark field microscope.

iii) Fluorescent microscope.

3.4 Micrometery : Basic pinciples, uses & application.

3.5 Stains and dyes.

i) Definition : stain, dye, chromogen, chromophore, auxochrome, acidic and basic stains, simple and differential staining. (Gram’s and Acid fast staining ) , natural stains, mordant, decolourizer, counter stains.

ii) Brief survey of classification of dyes, knowledge of group & stains used in practical course of B.Sc. I. Physicochemical basis of staining.

iii) Fixatives and fixation of smears.

iv) Staining of Fungi.

v) Use & significance of stains in Microbiology.
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