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IBGE- 303 Plant Tissue Culture 1st semester 4(3-2)



Theory

Introduction to plant tissue culture, history, explants, choice of explants, role of explants in callus and suspension cultures and micro propagation, culture media, choice of media, nutritional components of tissue culture media, induction and initiation of callus culture, subculture and maintenance, types of cultures, measurement of growth (callus and suspension culture), stages of cell growth, factors affecting callus growth, organogenesis, embryogenesis and protoplast.

Practical


Laboratory regulation, safe handling and use of lab equipments (pH meter, orbital shaker, autoclave, laminar flow unit , and growth chamber) glass wares and chemicals, aseptic techniques.

Recommended Books

  • Principles of Tissue Engineering. R.P Lanza, R. Langer, and J Vacanti. 2000. 2nd Edition. Academic Press, California.

  • Plant Tissue Culture Manual, K. Lindesey, (19970, Kluwer Academic Publisher, Netherlands



Eng-301 Structure and Writing Skills 1st Semester 3(2-2) credits

Course contents same as included in the prospectus for B. Sc (Hons) Agriculture

Math-301 Maths-I 1st semester 3(2-2) credits



Course contents same as included in the prospectus for B. Sc (Hons) Agriculture


IBGE-311 Cell and Tissue culture 2nd semester 4(3-2)



Theory

Totipotency, differentiation and meristems in plants, regeneration of organ and embryos, protoplast isolation methods, protoplast culture, protoplast fusion, selection for somatic hybrids, uses of plant tissue cultures: mcropropogation, production of virus free plant, clonal propogation, embryo rescue and ecbryo culture, somatic hybridization, haploid cell culture, anther culture, pollen culture, secondary metanbolite production, cryopreservation, somaclonal variation (mtant selection in vitro).

Practical


Culture media, composition media, preparation of stock solution, preparation of semisolid and suspension culture media, sterilization, methods of sterilization for media and equipments sterilization of explant, inoculation of explants, incubation of explants, induction of callus an suspension cultures.
Recommended Books

  • Principles of Tissue Engineering. R.P Lanza, R. Langer, and J Vacanti. 2000. 2nd Edition. Academic Press, California.

  • Plant Tissue Culture Manual, K. Lindesey, (19970, Kluwer Academic Publisher, Netherlands



IBGE-312 Biotechnology-II 2nd Semester 4 (3-2) Credits


THEORY:


The organism and relationship between primary and secondary metabolism, cell cycle (bacterial growth).


History and application of genetic engineering; genome, management and analysis, basic tools of genetic engineering (isolation, cutting, ligation etc); mechanisms of gene transfer; DNA sequencing and genome libraries.


Introduction to nature, classification and action; application of enzymes in genetic and protein engineering. Introduction to role of enzymes in industrial product synthesis.


Cell tissue culture details of culture techniques (explant, sterilization, growth, differentiation, etc), applications.


Principles of microbial growth; important products formation. bioreactor/fermenter technology .


Pharmaceuticals and bio-pharmaceuticals, antibiotics, vaccines etc. Introduction to gene therapy and AIDS.


Recommended Books


  • Molecular Biology, Daved Freifelder, Second Edition (1987), Jones & Barlet Publisher,Boston.

  • Molecular Biology and Biotechnology, J.M. and Walkar and E,B, Gingold, third Edition(1993), Royal Chemistry Society



IBGE-313 CELL BIOLOGY 2nd Semester 3 (2-2) Credits


THEORY:


Introduction to cell theory and structure; chemical composition of cell; types of cell organelles, structure and their functions; separation of cell organelles; transport properties of cell membrane; cell cycle; mitosis; molecular organization and functional role; meiosis, division and genetic consequences; comparison with mitosis, chromosomal aberrations, variation in chromosome number and structure; material basis of heredity; chromosomes of bacteria, viruses and their functions.


Genes, chromosomes and the mechanism of Mendelian inheritance, multiple alleles and gene interaction, epistasis, pleiotropy, sex determination and differentiation; sex-linked inheritance, sex-linked genes, sex anomalies; linkage and crossing over; linkage analysis and gene mapping in eukaryotes.
PRACTICAL:


Microscopic examination of prokaryotic and eukaryotic cells; preparation of slides to show stages of cell division; study of some properties of biological molecules. Study of cell structure and division; separation and characterization of cell organelles


Recommended Books


  • Cell and Molecular Biology, Karp, 3rd Edition. 2002. John Wiley and Sons.

  • Molecular Biology of the Cell, B. Alberts. D. Bray, J. Lewis. M. Raff, K. Roberts and J.D. Watson, Third Edition(1994), Garland Publishers.



Math- 311 Maths-II 2nd semester 3 (3-0) credits


Course contents same as included in the prospectus for B. Sc (Hons) Agriculture


CS/IT-311 Computer Science/Information Technology 2nd semester 3(2-2)credits


Course contents same as included in the prospectus for B. Sc (Hons) Agriculture


ISES-301 Islamic Studies or Ethics 2nd semester 2(2-0) credits


Course contents same as included in the prospectus for B. Sc (Hons) Agriculture


IBGE-401 ELEMENTS OF BIOTECHNOLOGY 3rd Semester 4(3-2)Credits


THEORY:


Introduction to biotechnology; microbial growth kinetics; shake-flask, solid state, anaerobic, batch and continuous fermentations; pilot scale and scaling up of fermentations; bioinstrumentation and computer control of fermentation processes; immobilized enzymes and cells; mammalian and plant cell culture; industrially important micro-organisms and biotechnological products; economic aspects of fermentation processes; biotechnology in health, agriculture and industry; future trends in biotechnology.

PRACTICAL:


Preparation and characterization of chromosomal, plasmid and bacteriophage DNA and RNA; mini project to clone a specific gene in E. coli; techniques used in molecular biology and genetic engineering.


Recommended Books


  • Principle of Plant Biotechnology: an introduction to genetic engineering in plants. S.H. Mantel, J.A. Mathews, R.A. Mckee (9185), Blackwell Scientific Publication, Oxford, London, Boston.

 Biotechnology in 21st Century, Ayyana, C. (1993), McGraw Hill


IBGE-402 Biological Chemistry-I 3rd Semester 4 (3-2) Credits


THEORY:


1 Amino Acids & Proteins

Introduction, Classification of amino acids, peptide linkage, chemical properties of amino acid, importance of proteins, Classification of proteins (simple, compound and derived proteins), Structure of proteins, primary structures & its determination by Edman reaction, secondary statures (a-helix and B-sheets), tertiary structure and globular proteins, quaternary structures, Functions, separation and biological significance of amino acids & proteins.


2. Vitamins & Micro/macronutrients

Introduction, Classification of Vitamins, Fat soluble (Vitamin A, D, E, & K) and Water Soluble (Thiamin, Riboflavin, Niacin and Ascorbic acid), sources, chemical structure, biological functions. Nutrients, classification, Macro & Micronutrients and their functions.
PRACTICAL:


Hydrolysis of a protein and qualitative tests for amino acids; paper chromatography of amino acids; estimation of proteins by CBB dye-binding, Lowry’s and Kjeldahl’s methods. Separation and quantification of vitamins from plants by HPLC.


Recommended Books


  • Fundamentals of Biochemistry, D. Voet, J.G. Voet, C. W. Pratt. 1999. John Wiley & Sons, New York.

  • Principles of Biochemistry, A. L. Lehninger, D.L. Nelson and M.M. Cox, 3rd Edition. 2000. Worth Publishers, New York.



IBGE-403 MOLECULAR BIOLOGY 3rd semester 4 (3-2) Credits



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