Chapter 5 Visualizing cells and the csk visible Light microscopy




Скачать 290.5 Kb.
НазваниеChapter 5 Visualizing cells and the csk visible Light microscopy
страница5/6
Дата28.09.2012
Размер290.5 Kb.
ТипДокументы
1   2   3   4   5   6

4. Flow Cytometry



Some applications in cytomechanics require high throughput analysis of cells and their content. FC can analyze aspects of cell structure and mechanical makeup at the rate of 10,000 cells per minute.




Figure 5.11 shows scatter plots for ECs exposed to isotonic and 50% hypotonic saline for 1 minute and more than an hour. The scatter plots show a dot for each cell event, with coordinates representing forward and side scatter of light as labeled. Approximately 10,000 cells are represented in each series. The side and forward scatter results seen at one minute for the isotonic control and 50% hypotonic controls were essentially unchanged when retested after more than an hour incubation in either set of control conditions. Hypotonic saline exposure for one minute increased side scatter.


Figure 5.11: Testing effects of drugs on mechanical response of endothelial cells.

5. Exercises





  1. How can the excitation light be separated from fluorescence in a microscope?

  2. From what you know of physiology, what mechanical role could a Ca++ wave play in the cell?

  3. Explain how confocal optics can “see” through thick objects.



6. References




Principles of Fluorescence Detection


Albani, J.R., Absorption et fluorescence: Principes et applications, Lavoisier (2001). This book is the first on absorption and fluorescence to be published in the French language.

Brand, L. and Johnson, M.L., Eds., Fluorescence Spectroscopy (Methods in Enzymology, Volume 278), Academic Press (1997).

Cantor, C.R. and Schimmel, P.R., Biophysical Chemistry Part 2, W.H. Freeman (1980) pp. 433–465.

Dewey, T.G., Ed., Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, Plenum Publishing (1991).

Guilbault, G.G., Ed., Practical Fluorescence, Second Edition, Marcel Dekker (1990).

Lakowicz, J.R., Ed., Topics in Fluorescence Spectroscopy: Techniques (Volume 1, 1991); Principles (Volume 2, 1991); Biochemical Applications (Volume 3, 1992); Probe Design and Chemical Sensing (Volume 4, 1994); Nonlinear and Two-Photon Induced Fluorescence (Volume 5, 1997); Protein Fluorescence (Volume 6, 2000); DNA Technology (Volume 7, 2003); Plenum Publishing.

Lakowicz, J.R., Principles of Fluorescence Spectroscopy, Second Edition, Plenum Publishing (1999).

Mathies, R.A., Peck, K. and Stryer, L., "Optimization of high-sensitivity fluorescence detection," Anal Chem 62, 1786–1791 (1990).

Powe, A.M., Fletcher, K.A., St. Luce, N.N., Lowry, M., Neal, S., McCarroll, M.E., Oldham, P.B., McGown, L.B. and Warner, I.M., "Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry," Anal Chem 76, 4614–4634 (2004).

Royer, C.A., "Approaches to teaching fluorescence spectroscopy," Biophys J 68, 1191–1195 (1995).

Sharma, A. and Schulman, S.G., Introduction to Fluorescence Spectroscopy, John Wiley and Sons (1999).

Valeur, B., Molecular Fluorescence: Principles and Applications, John Wiley and Sons (2002).
1   2   3   4   5   6

Похожие:

Chapter 5 Visualizing cells and the csk visible Light microscopy iconBiology of Cells: Prokaryotic and Eukaryotic cells E. coli and Yeast; Cell organelles and

Chapter 5 Visualizing cells and the csk visible Light microscopy iconBiology of Cells: Prokaryotic and Eukaryotic cells E. coli and Yeast; Cell organelles and their functions, Differences and similarities in plant and animal

Chapter 5 Visualizing cells and the csk visible Light microscopy icon[1] S. K. Chapman, The Surface Image: a scanning Electron Microscopy Revolution, European Microscopy and Analysis, May 1992, 15-17; [2]
Наставник или наставници (презиме, средње слово име): Маричић М. Алекса, Симеуновић Љ. Радојко
Chapter 5 Visualizing cells and the csk visible Light microscopy iconEnvironmental effects of artificial light and light pollution

Chapter 5 Visualizing cells and the csk visible Light microscopy iconThe light is dim as you enter the room after receiving no answer from repeated knocking. The lack of light is due to aluminum foil and newsprint taped to the

Chapter 5 Visualizing cells and the csk visible Light microscopy iconSnapshot: Preface to visualizing the future

Chapter 5 Visualizing cells and the csk visible Light microscopy iconStudents studying for Bio U117 Exam 1 should only review the sections «Introduction and terminology», «Basic chemistry», «Cells and membrane transport
...
Chapter 5 Visualizing cells and the csk visible Light microscopy iconRecommendations for action the Search for Visible Unity between the Churches

Chapter 5 Visualizing cells and the csk visible Light microscopy iconChapter 2 70 Effect of novel nop receptor ligands on ethanol drinking in the alcohol-preferring msP rats Chapter 3 96

Chapter 5 Visualizing cells and the csk visible Light microscopy iconService statute, in Title 42 Chapter 6A to Chapter 1, to repeal

Разместите кнопку на своём сайте:
Библиотека


База данных защищена авторским правом ©lib.znate.ru 2014
обратиться к администрации
Библиотека
Главная страница