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4. Flow Cytometry
Some applications in cytomechanics require high throughput analysis of cells and their content. FC can analyze aspects of cell structure and mechanical makeup at the rate of 10,000 cells per minute.
Figure 5.11 shows scatter plots for ECs exposed to isotonic and 50% hypotonic saline for 1 minute and more than an hour. The scatter plots show a dot for each cell event, with coordinates representing forward and side scatter of light as labeled. Approximately 10,000 cells are represented in each series. The side and forward scatter results seen at one minute for the isotonic control and 50% hypotonic controls were essentially unchanged when retested after more than an hour incubation in either set of control conditions. Hypotonic saline exposure for one minute increased side scatter.
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| S. K. Chapman, The Surface Image: a scanning Electron Microscopy Revolution, European Microscopy and Analysis, May 1992, 15-17; |
Наставник или наставници (презиме, средње слово име): Маричић М. Алекса, Симеуновић Љ. Радојко
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