Curriculum and Syllabus for M. Tech. Industrial Biotechnology (revised in 2010 (bos)




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Dr. M.G.R. University


Dept. of Biotechnology


Curriculum and Syllabus for M.Tech. Industrial Biotechnology (revised in 2010 (BOS)


Preamble

  1. This course will offer specialization and hands on practical training with a potential for job placement in Biotechnology Industry.

  2. Eligibility: Candidates with an M.Sc. In Biotechnology, Botany, Zoology, Microbiology, Biochemistry or any of Life Science oriented subjects; 4-year professional courses such as B.Tech. Biotechnology, Industrial Biotechnology, Chemical Engineering, B.Pharm. B.V.Sc. or M.B.B.S. or Agriculture.

  3. The total credits for this course will be 70.

4.    The first Semester will teach the basic papers required for Biotechnology.

Bridge courses: A Chemical Engineering basic paper is mandatory for M.Sc. students; Chemical Engineering and B.Pharm candidates must take Genetics paper.   The others may opt for a Maths course;

  1. Intensive Laboratory courses in Biochemical Techniques and Microbial Techniques will be offered in the first Semester.

  2. In the Second Semester, courses will be offered in application oriented papers. There will be 4 core papers and 1 Electives followed by 2 Labs.

7.    In the third Semester, the candidates will take 3 core and 2 elective papers and 1 lab course.   This Semester will include a project pre-preparation and literature review.

8.    The last Semester will be entirely a project which will be marked based on a viva voce and Project report.

9. The same course can also be offered in Part-time mode, where the course will be spread over 6 Semesters.

10. If required students may opt for additional credits from other Dept.   However they will be included as a criteria for passing M.Tech. Industrial Biotechnology only if approved by the Board that this is considered part of Biotechnology.


Curriculum for M.Tech. Industrial Biotechnology (Full Time)

Credit Distribution


Semester

Credits

I

22

II

22

III

22

IV

9

TOTAL

75



I Semester

S.No.

Code

Course

L

T

P

C

1

MBT101

Advanced Microbiology

3

1

0

4

2

MBT103

Biochemistry and Enzymology

3

1

0

4

3

MBT105

Instrumentation Methods

3

0

0

3

4

MBT107/109

Cell & Molecular Biology

3

1

0

4




6

MBT111

Biochemical Techniques Lab

0

0

6

2

7

MBT113

Microbial Techniques Lab

0

0

6

2

Bridge Courses (any one will be given)

8

MBTB101

Genetics

3

0

0

3




MBTB103

Basic Principles of Chemical Engineering

3

0

0

3




MBTB105

Mathematics

3

0

0

3







TOTAL

15

3

12

22


II Semester

S.No.

Code

Course

L

T

P

C

9

MBT102

Genetic Engineering

3

1

0

4

10

MBT104

Bioinformatics

3

1

0

4

11

MBT106

Quantitative Methods

3

0

0

3

12

MBT108

Fermentation Technology

3

1

0

4




13

MBT110

Genetic Engineering Lab

0

0

6

2

14

MBT112

Fermentation Technology Lab

0

0

6

2




Electives – Any One will be offered (15)




MBTE02

Biology of the Immune System

3

0

0

3




MBTE04

Food Technology

3

0

0

3




MBTE06

Developmental Biology

3

0

0

3




MBTE08

Advances in Agricultural Biotechnology

3

0

0

3




MBTE10

Pharmaceutical Technology

3

0

0

3




MBTE12

Environmental Biotechnology

3

0

0

3







TOTAL

15

3

12

22



III Semester

S.No.

Code

Course

L

T

P

C

16

MBT201

Genomics and Proteomics

3

1

0

4

17

MBT203

Animal and Plant Tissue Culture

3

1

0

4

18

MBT205

Intellectual Property Rights in Biotechnology

3

0

0

3

19

MBT211

Seminar

0

1

0

1




20

MBT207

Tissue culture Lab

0

0

6

2

21

MBT209

Project Literature Review and Project Proposal

0

0

6

2

Electives –Two will be offered (22 – 23)




MBTE211

Separation Techniques

3

0

0

3




MBTE213

Biotechnology of Animal Production

3

0

0

3




MBTE217

Stem cells in Healthcare

3

0

0

3




MBTE231

Bio Business

3

0

0

3







TOTAL

15

3

12

22

IV Semester

S.No.

Code

Course

L

T

P

C

24

MBT202

Project Report and Viva voce

0

0

24

9







TOTAL

0

0

24

9



SYLLABUS-I Semester


MBT101 Advanced Microbiology 3 1 0 4


Unit 1: New approaches to Bacterial taxonomy, determination and significance of DNA

Base composition, nucleic acid hycridization, RNA fingerprinting, bacterial phylogeny.


Unit 2: Metabolic diversity of aerobic heterotrophs – mechanisms in uptake of substrates

Entner-Doudoroff pathway, sugar degradation via Pentose Phosphate cycle, methyl glyoxal bypass, diversity in energy metabolism.


Unit 3: Bacterial Fermentation – Alcohol fermentation, lactate fermentation, buatyrate &

butanol –acetone fermentation, Anaerobic food chains; Chemolithotrophic and phototropic metabolism.


Unit 4: Degradation of natural substances – Cellulose degradation, microbial conversion

in the rumen, xylan degradation, degradation of starch, fructans, mannan, pectin, agar, chitin, lignin; formation of humus, utilization of hydrocarbons – methane, ethane, propane, butane, aromatic hydrocarbons, xenobiotics.


Unit 5: Biodeterioration control and soil, waste and water management – Indicator microorganisms, fouling biofilms, treatment of solid waster, landfills, composting, treatment of liquid waste, biological oxygen demand.


References:

  1. General Microbiology, Fifth edition, (2006), Stanier RY, Ingraham JL, Wheels ML and RP Painter, Macmillan Press.

  2. Bacterial Metabolism, 2nd Edition (1986) Gerhard Gottschalk, Springer Verlag.

  3. Microbial Ecology – Fundamentals and Applications, 4th Edition, (2005), Atlas RM and R Bartha.

  4. General Microbiology, 7th Edition (1992), Hans G. Schlegel, Cambridge University Press.



MBT103 BIOCHEMISTRY AND ENZYMOLOGY 3 1 0 4

UNIT1. INTRODUCTION TO BIOMOLECULES 9hrs Structure and properties of Mono, Di, Oligo and polysaccharides, complex carbohydrates, Structure and properties of Fatty acids, Glycerolipids, phospholipids, sphingolipids, glycolipids, steroids, Structure and properties of amino acids, Peptides, proteins and conjugated proteins. Structure and properties of purines, pyrimidines, nucleosides, nucleotides, polynucleotides, Ribonucleic acids and deoxy ribonucleic acids,

nucleoprotein complexes.

UNIT 2. METABOLISM AND INTERMEDIARY METABOLISM 10hrs Biosyntheses and degradation of fatty acids and cholesterol, Biosyntheses and degradation of amino acids, peptides and proteins; Biosynthesis and degradation of amino acids, peptides and proteins, Biosyntheses and degradation of Purines, pyrimidines and nucleic acids.TCA Cycle, glycolysis, gluconeogenesis, Pentose phosphate shunt, Embden Meyerhof pathway, urea cycle, interconnection of pathways, Metabolic regulation, Bioenergetics: Respiratory chain, TP cycle, energy rich compounds .

UNIT 3.ENZYME KINETICS 10hrs

Classification,Nomenclature, Enzyme Kinetics –Michaelis Menton Equation Isoenzymes –Coenzymes –Active site Iinhibitor-Affecting factors of enzyme activity. Specificity of enzymes: Type of specificity, The Koshland “induced fit” hypothesis, Strain or transition – state stabilization Hypothesis. Enzyme catalysis and kinetics: Factors affecting the rate of chemical reaction, Kinetics of unanalyzed chemical reaction, Kinetics of enzyme – catalyzed reaction, Methods for investigating the kinetics of enzyme catalyzed reactions, Nature of enzyme catalysis, inhibition of enzyme activity.

UNIT 4. ENZYME ACTIVE SITE 8hrs

The investigation of active site structure and chemical nature of enzyme catalysis: The identification of binding sites and catalytic site, Three dimensional structure of active site, Mechanism of catalysis,Mechanism of reaction catalyzed by enzyme without cofactor, Metal activated enzyme and metalloenzyme, Coenzymes in enzyme catalyzed reactions.

UNIT 5.APPLICATIONS OF ENZYMES 8hrs

Additional industrial enzymes: lipases, Penicillin acylase, Amino acylase & amino acid production, Cyclodextrin & Cyclodextrin glycosyl transferase, Enzymes in animal nutrition, Oxidoreductases, Enzymes in molecular biology Immobilization of enzymes: Concept, method of immobilization, Kinetics pf immobilized enzyme, effect of solute partition and diffusion on kinetics of immobilized enzyme, use of immobilized enzyme, Bioreactors using immobilized enzyme. Enzyme engineering: prediction of enzyme structure, design and construction of novel enzymes
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