Curriculum and Syllabus for M. Tech. Biotechnology




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Dr. M.G.R. University

Dept. of Biotechnology

Curriculum and Syllabus for M.Tech. Biotechnology

Curriculum for M.Tech. Biotechnology (Full Time)

Credit Distribution


Semester

Credits

I

22

II

22

III

22

IV

9

TOTAL

75



I Semester

S.No.

Code

Course

L

T

P

C

1

MBT 151

Human Physiology and Immunology

3

1

0

4

2

MBT103

Biochemistry and Enzymology

3

1

0

4

3

MBT105

Instrumentation Methods

3

0

0

3

4

MBT107/109

Cell & Molecular Biology

3

1

0

4

5

MBT165

Molecular Microbiology and Metagenomics

3

0

0

3




6

MBT111

Biochemical Techniques Lab

0

0

6

2

7

MBT113

Microbial Techniques Lab

0

0

6

2







TOTAL

15

3

12

22



II Semester

S.No.

Code

Course

L

T

P

C

8

MBT102

Genetic Engineering

3

1

0

4

9

MBT104

Bioinformatics

3

1

0

4

10

MBT106

Quantitative Methods

3

0

0

3

11

MBT 156

Human Genomics

3

1

0

4

ELECTIVES-Any One Will be Offered(12)




MBTE04

Food Technology

3

0

0

3




MBTE10

Pharmaceutical Technology

3

0

0

3




MBTE 160

Immunotechnology

3

0

0

3




13

MBT110

Genetic Engineering Lab

0

0

6

2

14

MBT 162

Bioinformatics Lab

0

0

6

2







TOTAL

15

3

12

22



III Semester

S.No.

Code

Course

L

T

P

C

15

MBT 251

Proteomics

3

1

0

4

16

MBT 253

Animal Tissue Culture

3

1

0

4

17

MBT 205

Intellectual Property Rights, Biosafety, Bioethics

3

0

0

3

18

MBT 229

Plant Molecular Breeding

3

1

0

4

Electives –One will be offered (19)




MBTE 219

Phytochemistry

3

0

0

3




MBTE 221

Clinical Research

3

0

0

3




MBTE 223

Stem cells Technology

3

0

0

3




MBTE 225

Cancer Biology

3

0

0

3




20

MBT 207

Tissue culture Lab

0

0

6

2

21

MBT211

Seminar

0

0

6

2







Total

15

3

12

22



IV Semester

S.No.

Code

Course

L

T

P

C

22

MBT 202

Project Report and Viva voce

0

0

24

9







TOTAL

0

0

24

9



I semester

MBT 151 Human Physiology and Immunology 3 1 0 4

Unit 1: INTRODUCTION AND PHYSIOLOGY OF CELLS Foundations of Physiology – Functional Organization of the cell – Physiology of Membranes – Signal Transduction –Electrophysiology of the Cell Membrane – Electrical Excitability and Action Potentials – Synaptic Transmission and the Neuromuscular Junction – Cellular Physiology of Skeletal, Cardiac and Smooth Muscle, Arteries Veins and Microcirculation – Heart pump – regulation of arterial pressure and cardiac output


Unit 2: RESPIRATORY, URINARY AND GASTROINTESTINAL SYSTEMS

Organization of Respiratory system –Transport of Oxygen and Carbon dioxide in the Blood – Gas Exchange in the Lungs – Ventilation and Perfusion of the lungs, Organization of Urinary System – Glomerular filtration and Renal Blood flow – Transport of salts, organic solutes and acids and bases – Urine Concentration and Dilution – Organisation of Gastrointestinal system – Gastric function – Pacreatic and salivary glands – Nutrient digestion and Absorption.


Unit 3: ENDOCRINE AND REPRODUCTIVE SYSTEMS

Organization of Endocrine control – Endocrine regulation and growth – Thyroid, Adrenal, Pancreas and Parathyroid glands – Sexual differentiation – Male and Female Reproductive systems – Fertilization – Pregnancy and Lactation


Unit 4: Molecular Immunology: Cells of immune system; innate and acquired immunity, Development, maturation, activation and differentiation of T-cells and B-cells; TCR; antibodies: structure and functions; antibodies: genes and generation of diversity; antigen-antibody reactions; monoclonal antibodies: principles and applications; antigen presenting cells; major histocompatibility complex; antigen processing and presentation; regulation of T-cell and B-cell responses.


Unit 5 : Infection, Immunity and Transplantation:

Properties of Cytokines, cytokine secretion by Th1 and Th2 subsets, Complement activation, Inflammation, Acute phase response, Immune response to infectious diseases, Hypersensitivity, genetics of transplantation, Autoimmune diseases.

TOTAL : 45

TEXT BOOK
1. Sarada Subramanyam, K.Madhavan Kutty and H.D.Singh – Text book of ‘Human

Physiology’ – S.Chand & Company, 1996.

2. Sujit K.Chaudhuri – Concise Medical Physiology – New Central Book agency, 1997.

3. Kuby- immunology (4th edition) by R.A Goldsby, T.J Kindt, B.A. Osborne

4. Essentials of immunology (6th edition): Ivan Riot- Blakswell scientific publications, Oxford, 1988.

REFERENCES
1. Arthur.C.Guyton – Textbook of Medical Physiology – Prism Book (p) Ltd. 1996.


2. Cyril A.Keele Eric Neil and Neil Norman Joels Samson Wrigths’ Applied Physiology –

Oxford University Press – 1983.


3. Fundamentals of immunology: Paul W. E. (Eds.) Raven press, New York, 1988


MBT103 BIOCHEMISTRY AND ENZYMOLOGY 3 1 0 4

UNIT1. INTRODUCTION TO BIOMOLECULES 9hrs Structure and properties of Mono, Di, Oligo and polysaccharides, complex carbohydrates, Structure and properties of Fatty acids, Glycerolipids, phospholipids, sphingolipids, glycolipids, steroids, Structure and properties of amino acids, Peptides, proteins and conjugated proteins. Structure and properties of purines, pyrimidines, nucleosides, nucleotides, polynucleotides, Ribonucleic acids and deoxy ribonucleic acids,nucleoprotein complexes.

UNIT 2. METABOLISM AND INTERMEDIARY METABOLISM 10hrs Biosyntheses and degradation of fatty acids and cholesterol, Biosyntheses and degradation of amino acids, peptides and proteins; Biosynthesis and degradation of amino acids, peptides and proteins, Biosyntheses and degradation of Purines, pyrimidines and nucleic acids.TCA Cycle, glycolysis, gluconeogenesis, Pentose phosphate shunt, Embden Meyerhof pathway, urea cycle, interconnection of pathways, Metabolic regulation, Bioenergetics: Respiratory chain, TP cycle, energy rich compounds .

UNIT 3.ENZYME KINETICS 10hrs Classification,Nomenclature, Enzyme Kinetics –Michaelis Menton Equation Isoenzymes –Coenzymes –Active site Iinhibitor-Affecting factors of enzyme activity. Specificity of enzymes: Type of specificity, The Koshland “induced fit” hypothesis, Strain or transition – state stabilization Hypothesis. Enzyme catalysis and kinetics: Factors affecting the rate of chemical reaction, Kinetics of unanalyzed chemical reaction, Kinetics of enzyme – catalyzed reaction, Methods for investigating the kinetics of enzyme catalyzed reactions, Nature of enzyme catalysis, inhibition of enzyme activity.

UNIT 4. ENZYME ACTIVE SITE 8hrs The investigation of active site structure and chemical nature of enzyme catalysis: The identification of binding sites and catalytic site, Three dimensional structure of active site, Mechanism of catalysis,Mechanism of reaction catalyzed by enzyme without cofactor, Metal activated enzyme and metalloenzyme, Coenzymes in enzyme catalyzed reactions.

UNIT 5.APPLICATIONS OF ENZYMES 8hrs Additional industrial enzymes: lipases, Penicillin acylase, Amino acylase & amino acid production, Cyclodextrin & Cyclodextrin glycosyl transferase, Enzymes in animal nutrition, Oxidoreductases, Enzymes in molecular biology Immobilization of enzymes: Concept, method of immobilization, Kinetics pf immobilized enzyme, effect of solute partition and diffusion on kinetics of immobilized enzyme, use of immobilized enzyme, Bioreactors using immobilized enzyme. Enzyme engineering: prediction of enzyme structure, design and construction of novel enzymes
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