Uws laboratory Safety Guidelines




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UWS Laboratory Safety Guidelines


These guidelines were prepared by the University of Western Sydney Occupational Health Safety and Risk Management Unit.


UWS Laboratory Safety Guidelines

Table of Contents





1 Introduction 1

Glossary 1

2 Responsibilities 1

2.1 All Staff (permanent and casual) and Students 1

2.2 Deans and Heads of School and/or Department 2

2.3 Academics (Course, Unit and/or Local Unit Coordination) 2

2.4 Academics (permanent or casual) with teaching responsibilities 2

3.5 Researchers 3

3.6 Research Supervisors 4

3.8 Technical Staff (permanent and casual) 5

3.9 Capital Works & Facilities Staff 6

3.10 Undergraduate Students 7

3.11 Honours and Postgraduate Students 7

3.12 Contractors 8

3.13 Visitors 8

3.14 References 8

3 Security Management 1

4.1 Introduction 1

4.2 Key Aspects of Security Management 1

4.2.1 People 1

4.2.2 Buildings 2

4.2.3 Contents of Buildings 2

4.3 Reporting Breaches of Security 3

4 Emergency Management 1

5.1 Introduction 1

5.2 Responsibility for Developing, Implementing & Testing of Plans 1

5.3 Staff Involvement in Implementing Emergency Management Plans 2

5.4 Testing of Laboratory Emergency Management Plans 2

5.5 Emergency Contact Numbers 2

5.6 Dangerous Goods Emergencies 3

5.7 Needle-Stick Injuries and Other Biological Hazard Exposures 4

5.7.1 Introduction 4

5.7.2 Management of Needle Stick & Biological Exposures 4

5.8 Spills Management 5

5.8.1 General Requirements 5

5.8.2 Emergency Spill Procedure 5

5.8.3 Maintenance and Supply of Spill Kits 6

5.8.4 Chemical Spills 6

5.8.4.1 Chemical Spills in Fume Cupboards 8

5.8.5 Mercury Spills 8

5.8.6 Radiation Spills 9

5.8.7 Biological Spills 9

5.8.7.1 Introduction 9

5.8.7.2 Microorganisms 10

5.8.7.3 Spills in Biosafety Cabinets 11

5.8.7.4 Human Blood and Body Fluids 12

5.8.7.5 Spills in Centrifuges 13

5.9 References 14

5 First Aid 1

6 Safety Equipment 1

7.1 Introduction 1

7.2 Fixed Safety Equipment 1

7.2.1 Safety Showers and Eyewash Facilities 1

7.3 Fire Extinguishers, Fire Blankets and Fire Hoses 2

7.3.1 Introduction 2

7.3.2 Servicing, Checking and Replacement 2

7.3.3 Signage and Placards 2

7.4 Portable Safety Equipment 2

7.4.1 Personal Protective Equipment (PPE) 3

7.4.2 Supply & Maintenance of PPE 4

7.5 References 4

7 Risk Management 1

7.1 Introduction 1

7.2 Laboratory Risk Assessment & Control Protocol 1

7.3 Sample Laboratory Risk Assessments (LRAs) 2

7.4 Risk Assessments for Undergraduate Students 2

7.5 Risk Assessments for Hazardous Substances and Dangerous Goods 2

8.6 References 3

8 Laboratory Safety Inductions 1

8.1 Introduction 1

8.2 Undergraduate Students 1

9.3 Staff and Postgraduate Students 1

9.4 Casual Staff 2

9.5 Contractors Working Within Laboratories 2

9.6 Visitors 2

9.7 References 2

9 General Laboratory Safety 1

9.1 General Rules and Regulations 1

9.2 Access to Laboratories and Associated Facilities 1

9.3 Regulations for After Hours Work 2

9.4 Working Alone or In Isolation 2

10.4.1 Introduction 2

10.4.2 Risk Control 3

10.5 Unattended Work In Progress 5

10.6 Overnight Work In Progress 5

10.7 Housekeeping 5

10.8 Glassware (General) 6

10.9 Handling and Disposal of Sharps 7

10.9.1 Introduction 7

10.9.2 Broken Glass (Clean and Contaminated) 7

10.9.3 Handling and Disposal of Other Sharps 8

10.9.4 Injury with Sharps Contaminated with Blood or Other Biological Material 8

10.10 References 10

10 Gas Cylinders 1

10.1 Introduction 1

10.2 General Precautions 1

10.3 Storage Facilities 1

10.4 Moving Gas Cylinders 1

10.5 Indoor Storage of Gas Cylinders 2

11.6 References 3

Cryogenic Fluids 1

12.1 Introduction 1

12.2 General Procedures 1

12.3 Storage 2

12.4 Transferring Cryogenic Fluids 2

12.5 Working at Reduced Pressure 3

12.6 Special Precautions 3

12.7 References 4

Chemical Safety 1

13.1 Introduction 1

13.2 Register of Hazardous Substances and Dangerous Goods 1

13.2.1 Introduction 1

13.2.2 Master Register 1

13.2.3 Local Registers 2

13.3 Material Safety Data Sheets 2

13.3.1 Introduction 2

13.3.2 Obtaining a MSDS 2

13.3.3 Updating a MSDS 3

13.4 Risk Assessments 3

13.4.1 Introduction 3

13.4.2 Risk Assessments for Hazardous Substances 4

13.4.3 Risk Assessments for Dangerous Goods 4

13.5 Labelling 5

13.5.1 Introduction 5

13.5.2 Protocols 5

13.6 Storage & Handling of Chemicals 6

13.6.1 Introduction 6

13.6.2 General Requirements 6

13.7 Induction and Training 7

13.8 Dangerous Goods 7

13.8.1 Introduction 7

13.8.2 Classification 8

13.8.3 Packing Groups 8

13.8.4 Legal Obligations 10

13.8.5 Plant, equipment and containers 10

13.9 Hazardous Substances 11

13.9.1 Introduction 11

13.9.2 Legal Obligations 11

13.10 Carcinogenic, Mutagenic and Highly Toxic Substances 12

13.10.1 Introduction 12

13.10.2 Legal Obligations 13

13.10.3 General Safety 14

13.10.4 Storage and Labelling of Carcinogens 15

13.10.5 Contamination with Carcinogens 16

13.10.6 Monitoring for Carcinogens 16

13.10.7 Disposal of Carcinogens 16

13.11 Poisons & Drugs 17

13.12 Regulation of Security Sensitive Ammonium Nitrate (SSAN) 17

13.12.1 Introduction 17

13.12.2 Licencing Requirements 18

13.12.3 Licencing Exemptions 19

13.13 Flammable Liquids 19

13.14 Chemical Spills 20

13.14.1 Introduction 20

13.15 References 21

11 Biological Safety 1

14.1 Introduction 1

1

14.2 Work Practices 2

14.3 Microorganisms 3

14.3.1 Introduction 3

14.3.2 Risk Groups 3

14.3.3 Physical Containment Levels 4

14.4 Genetic Manipulation Work 5

14.4.1 Introduction 5

14.4.2 UWS Biosafety and Radiation Safety Committee (BRSC) 5

14.5 Quarantine Biological Material 6

14.5.1 Introduction 6

14.5.2 Imported Biologicals 6

14.5.3 In vivo use of Imported Biologicals 7

14.5.4 Quarantine Approved Premises 7

14.6 Safe Handling of Blood, Body Fluids and Tissues (Human or Animal) 8

14.6.1 Introduction 8

14.6.2 Standard Operating Procedures (SOP) 8

14.7 Special Equipment 8

14.8 Disposal of Waste 9

14.9 Biological Spills 9

14.10 References 9

Radiation Safety 1

12 Disposal of Laboratory Wastes 1

16.1 Introduction 1

16.2 Waste Classification and Tracking Requirements 2

16.3 Responsibility for Laboratory Waste 4

16.4 Segregation of Laboratory Waste 4

16.5 Chemical and Solvent Waste 5

16.5.1 Introduction 5

16.5.2 Treatment of Chemical Waste 5

16.5.3 Segregation of Chemical Waste 5

16.5.4 Segregation of Carcinogenic and Cyanide Waste 5

16.5.5 General Procedures for Chemical Waste Storage and Disposal 6

16.6 Clinical and Biological Waste 6

16.7 Radiation Waste 10

16.8 Mixed Waste 10

16.9 References 11

13 Laboratory Animals 1

13.1 Introduction 1

13.2 Use of Laboratory Animals at UWS 1

13.3 References 2

14 Plant and Engineering 1

18.1 General Equipment 1

18.2 Fume Cupboards 1

18.2.1 Introduction 1

18.2.2 Design and Location 2

18.2.3 Maintenance and Testing 2

18.2.4 Guidelines Covering Effective Operation 3

18.2.5 Fume Cupboards for Use with Perchloric Acid 3

18.2.6 Fume Cupboards for Use with Hydrofluoric Acid 4

18.2.7 Risk Assessment for Use of Fume Cupboards 4

18.3 Autoclaves and other Pressure Equipment 5

18.3.1 Introduction 5

18.3.2 Safe Use of Pressure Equipment 6

18.3.3 Legal Obligations 7

18.3.4. Autoclaves for use in Biological/Microbiological Laboratories: 9

18.4 Biosafety Cabinets 10

18.4.1 Introduction 10

18.4.2. Classes of Biosafety Cabinets 11

18.4.3. General Requirements for Installation and Use of Biosafety Cabinets 12

18.5 Centrifuges 15

18.5.1 Introduction 15

18.5.2 Safety Requirements 16

18.6 Freeze-Dryers 16

18.6.1 Introduction 16

18.6.2 Safety Requirements 17

18.7 Refrigeration 17

18.8 Electrical Equipment 18

18.8.1 Introduction 18

18.8.2 Residual Current Devices in Laboratories 18

18.8.2.1 Legal Obligations 18

18.8.2.2 Labelling of RCD outlets 19

18.8.3 Tagging and Testing of Electrical Equipment 19

18.8.4 Maintenance of Records 20

18.9 Robotics 21

18.10 Machinery and Hand Tools 22

18.11 References 22

15 Nanotechnology 1

19.1 Introduction 1

19.2 Safety Considerations 2

19.3 Controls for Potential Nanotechnology Risks 2

19.4 References 3

16 Termination of Laboratory Work 1

20.1 Introduction 1

20.2 Termination of Laboratory Work Procedures for Hazardous Materials in Laboratories 1

20.2.1 Chemicals 2

20.2.2 Regulated Hazardous Substances (e.g. carcinogens, poisons) 2

20.2.3 Gas Cylinders 2

20.2.4 Animal and Human Tissue 2

20.2.5 Microorganisms and Cultures 3

20.2.6 Radioactive Materials 3

20.2.7 Mixed Hazards 3

20.2.8 Equipment 3

20.2.9 Shared Storage Areas 3

Further Information 1



Preface:


These guidelines have been designed to be used most effectively in an interactive format on-line. When accessed electronically, direct links are provided throughout the document for various internal and external references and sites. These links are underlined and are in blue font. Please also note that the links to the various Australian Standards throughout the guidelines will navigate only to the Australian Standards homepage. From this homepage a selection of the specific standard can be made. The ‘Table of Contents’ can also link directly to specific sections in the guidelines. These can be accessed by pointing on the selected section and following the instructions.


Separate guidelines for Radiation Safety are now available. Any matters relating to radiation safety will be covered in these guidelines, which will be available on the OHS website.

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